This page is an introduction to chromatography using thin layer chromatography as an example. Although if you are a beginner you may be more familiar with paper chromatography, thin layer chromatography is equally easy to describe and more straightforward to explain.
Thin-layer chromatography (TLC) is a very commonly used technique in synthetic chemistry for identifying compounds, determining their purity and following the progress of a reaction. It also permits the optimization of the solvent system for a given separation problem. In comparison with column chromatography, it only requires small quantities of the compound (~ng) and is much faster as well.
The European Pharmacopeia is moving away from the historical thin layer chromatography technique for the determination of Ninhydrin-positive substances, to more specific and sensitive methods utilising Liquid Chromatography (LC) or Amino Acid Analysers.
Cleaning capillary tubes with 35% H2O2 + muriatic acid mixture. From time to time we often run out of clean capillary tubes what are important if we would like to check our reactions and products purity with TLC (thin layer chromatography). The H2O2 and HCl mixture is a strongly oxidizing solution, what also dissolves gold (yes, not just aqua regia is suitable for this) and it also destroys everything what is organic, so it is perfect to clean glassware. Important note: elemental chlorine…