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What is shotgun sequencing?

Shotgun sequencing

Cannabis sativa cultivar Cannatonic, whole genome shotgun sequencing p - Nucleotide - NCBI

#ArnoldSchwarzenegger tore his skin several times filming the sequence cocking the shotgun in #Terminator2 (1991)

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Creative Biolabs is gladly to show a series of unrivalled De Novo Antibody Sequencing services, which have been developed based on our next generation antibody sequencing platform using the proprietary “Database Assisted Shotgun Sequencing" (DASS) technology. All species, isotypes and allotypes of antibodies can be sequenced with 100% coverage. Numerous successful cases from Creative Biolabs providing antibody http://www.creative-biolabs.com/next-generation-antibody-sequencing.html

Here, the author briefly introduces next-generation sequencing and then focuses on transcriptome shotgun sequencing (RNA-Seq). This article gives a broad overview and provides practical guidance for the many steps involved in a typical RNA-Seq work flow from sampling, to RNA extraction, library preparation and data analysis. He focuses on principles, presents useful tools where appropriate and point out where caution is needed or progress to be expected. This tutorial is mostly targeted at…

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Plant natural product research can be facilitated through genome and transcriptome sequencing approaches that generate informative sequence and expression datasets that enable characterization of biochemical pathways of interest. As the overwhelming majority of plant-derived natural products are derived from species with little, if any, sequence and/or genomic resources, the ability to perform whole genome shotgun sequencing and assembly has been and will continue to be transformative as…

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