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F4/80 antibody flow cytometry

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Oxford

f4/80 antibody flow cytometry

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Oxford

Excitation and Emission Spectra. Excitation and Emission Spectra of PI. Maximal excitation is 538 nm (blue) and maximal emission is 617 nm (red).

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A mixture of live and heat-killed Jurkat cells were stained with ReadiDrop Propidium Iodide for 1 min prior to sorting.

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Figure A. APC conjugated mouse anti human CD14 (MCA596APC) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. APC conjugated mouse anti human CD14 (MCA596APC) and FITC conjugated mouse anti human CD66b (MCA216F).

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Webinar: Optimize your Flow Cytometry Best practice for sample preparation, staining and analysis

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Figure A. Alexa®488 conjugated mouse anti human CD3 (MCA2184A488) and Alexa®647 conjugated Mouse IgG1 isotype control (MCA928A647). Figure B. Alexa®488 conjugated mouse anti human CD3 (MCA2184A488) and Alexa®647 conjugated mouse anti human CD4 (MCA1267A647).

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Figure A. RPE conjugated mouse anti mouse CD28 (MCA2473PE) and Alexa647® conjugated Rat IgG2a isotype control (MCA1212A647). Figure B. RPE conjugated mouse anti mouse CD28 (MCA2473PE) and Alexa647® conjugated rat anti mouse CD45 (MCA1258A647).

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Figure A. Alexa647® conjugated rat anti mouse CD94 (MCA2288PE) and RPE conjugated isotype control. Figure B. Alexa647® conjugated rat anti mouse CD94 (MCA2288PE) and RPE conjugated rat anti mouse CD161/NK1.1 (MCA1266PE).

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Figure A. RPE conjugated rat anti mouse CD4 (MCA4635PE) and APC conjugated Rat IgG2a isotype control (MCA1212APC). Figure B. RPE conjugated rat anti mouse CD4 (MCA4635PE) and APC conjugated rat anti mouse CD3 (MCA500APC).