Topoisomerase loosen the strands for unwinding, while meanwhile Helicase unwinds DNA at the replication fork. Binding Proteins and RNA primase form complex that initiates translation. DNA polymerase attaches to the initiation complex and begins transcribing Okazaki fragments that than are joined by DNA ligase. RNA primase initiates transcription. DNA Polymerase on the lagging strand forms the Okasaki fragments.
▶ DNA Replication: The Cell's Extreme Team Sport - YouTube. Ever wonder how all the new cells in your body get a copy of your DNA? DNA replication is a very intricate and fascinating process you don't want to miss. In this video, we'll give a brief overview of DNA replication for eukaryote cells and introduce 4 key players: ligase, DNA polymerase, primase, and helicase. We'll also talk about why DNA can either run 5' to 3' or 3' to 5' by exploring the structure of DNA.
DNA ligase, shown above repairing chromosomal damage, is an enzyme that joins broken nucleotides together by catalyzing the formation of an internucleotide ester bond between the phosphate backbone and the deoxyribose nucleotides.